Environmental metallomics laboratory

Our laboratory develops and applies approaches to environmental metallomics that are grouped into two departments:

1) Analyses of subcellular fractionation of trace metals in several biological materials (whole organism, liver, muscle, intestine) We expect to be able to optimize and adapt the protocol for subcellular fractionation of trace metals in biological samples. To this end, we are evaluating the influence of different homogenization strategies and certain cell fractionation parameters on the effectiveness of the protocol under study. Once optimized, the protocol will be applied to biological samples for the determination of trace metals.

2) Characterization of biomolecules targeted by these contaminants using coupling techniques. We are equipped to isolate and characterize metal-biomolecule complexes in various biological samples. Using coupling techniques, we combine the high separation power of chromatography (HPLC) with the high sensitivity and quantification accuracy of elemental mass spectrometry (ICP-QQQ). Depending on the metal under study, we develop multidimensional analytical strategies to characterize these complexes.

For more details : http://rosabal-laboratory.com

• Homogenizers (Pellet Pestle, Potter–Elvehjem, Polytron, Ultrason etc.)
• Microplate reader
• Ultracentrifuge
• ICP-QQQ
• Ultimate RSLCnano System

• Biological analyses

Matrix types

• Biological samples